Histomorphometric effects of energy drink consumption on pancreatic tissue of albino wistar rats.

Abstract

Objective: To determine the histo-morphometric effects of energy drink consumption in different doses on the pancreatic tissue of Wistar Albino rats. Study Design: Experimental study. Setting: Department of Pharmacology, Isra University, Hyderabad. Period: April 2021 to September 2021. Material & Methods: Thirty healthy albino Wistar rats of body weight 200±20 were included in the study. Rats were divided equally into three groups. Group I (Control), Group II (Low dose energy drink 7.5 ml/day) and Group III (High dose energy drink 15 ml/day). The body weight of all animals was measured. Blood samples were collected for the biochemical analysis including serum glucose, serum insulin level, and oxidative markers. Histo-pathological and morphometric findings were observed. Results: A statistically significant rise in body weight in groups II and III (p<0.05). Significantly increased serum glucose and declined insulin level was seen in group II and III compared with controls (p<0.05). There was a significantly lower level of oxidative markers observed in group III than in groups I and II (p<0.05). The mean pancreatic acini and islets diameter in groups II and III has decreased significantly compared with group I (p<0.05). The histo-pathological grading reveals that mild to moderate parenchymal changes in the pancreatic tissue of group II rats compared with group I. While moderate to severe changes were observed in group III compared with group I and II rats.  Conclusion: Caffeinated energy drink consumption results in oxidative stress and degenerative changes in the potential to seriously alter the exocrine and endocrine pancreas' normal morphology consumption.